Coding
Part:BBa_K2144007:Design
Designed by: Oskar hman Group: iGEM16_Stockholm (2016-10-12)
Coding sequence for Defensin with His6 and LPXTG-tag regulated by T7-promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 173
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The LT-sequence was inserted with Overhang PCR.
Source
-